期刊
CELLULAR IMMUNOLOGY
卷 320, 期 -, 页码 46-55出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2017.09.002
关键词
Cervical cancer cells; Adenosine; CTLs; CD73; Immunosuppression
资金
- CONACYT [240635]
- DGAPA-PAPIIT [IN217013, IN226516]
- Mexican Institute of Social Security, IMSS [957, 1161, 1014, 1613]
- Postdoctoral Scholarship Program of the UNAM
- postgraduate CONACyT fellowship [407865]
Background: The expression of CD73 in tumor cells plays a significant role in the production of adenosine (Ado) that suppresses antitumor effector cells. Methods: In this study we analyzed the capability of HPV-positive (HPV+) cervical cancer (CeCa) cell lines CaSki, SiHa, HeLa, and RoVa; and HPV-negative (HPV-) cell lines C33A and ViBo to produce Ado and inhibit effector functions of CD8+ T cells. Results: HPV+ CeCa cells expressed significantly higher levels of CD73 in the membrane (p < 0.01) than HPV-CeCa cells and this expression was associated with the production of larger amounts of Ado (> 400 mu M) compared to HPV-CeCa cells (< 200 mu M) in the presence of AMP, as well as a stronger inhibition of (> 50%) proliferation, activation, and cytotoxic activity of CD8+ T cells via interaction with A2A adenosine receptor. We also provide evidence that silenced E6/E7 expression in CeCa cells, strongly reduced its CD73 expression level and its capability to generate Ado. Conclusion: This results suggest that HPV infection, which is associated with more than 99% of CeCa cases, may present an increased constitutive expression of CD73 in cervical neoplasia to contribute to the suppression of the immune response mediated by the production of large amounts of Ado.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据