A highly selective fluorescent chemosensor for NADH based on calix [4]arene dimer

A new calix[4]arene dimer (C4D) has been designed and synthesized as a fluorescent chemosensor for the recognition of NADH in aqueous media. This chemosensor showed unique selectivity toward NADH over various biomolecules, with a detection limit of 24.5 nM (16.3 mu g L-1). Moreover, the stoichiometry of the C40-NADH complex formed was found to be 1:1 and the binding constant was calculated to be 3.74 x 10(5) M-1. (C) 2016 Elsevier Ltd. All rights reserved.