期刊
ACS OMEGA
卷 2, 期 12, 页码 8674-8677出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.7b01510
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资金
- EnginZyme AB
- KTH Royal Institute of Technology
Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
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