期刊
TALANTA
卷 165, 期 -, 页码 136-142出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.11.051
关键词
Carbon dots; MnO2 nanosheet; Alkaline phosphatase; Fluorescence
资金
- National Natural Science Foundation of China [21375076, 21205068, 21405094, 21505084]
- Key Research and Development Program of Shandong Province [2015GSF121031]
- Natural Science Foundation of Shandong Province [ZR2013BQ018, ZR2014BM029]
- Project of University-industry Cooperation of Jining City [2014018]
Using sterculia lychnophora seeds as precursors for the first time, fluorescent carbon dots (CDs) were synthesized by simple hydrothermal treatment. The quantum yield of as-synthesized CDs was 6.9% by using quinine sulfate as the reference. The fluorescence of CDs could be effectively quenched by a MnO2 nanosheet based on fluorescence resonance energy transfer (FRET). Ascorbic acid (AA) could reduce MnO2 to Mn2+ and result in the destruction of the MnO2 nanosheets, which could induce the fluorescence recovery of the CDs. In particular, alkaline phosphatase (ALP) could bio-catalyze acid 2-phosphate (AAP) hydrolysis to AA. Here, an efficient fluorescence probe based on a CDs-MnO2 nanosheet for rapid and selective detection of ALP was reported for the first time. Excellent performance for the detection of ALP was observed with high sensitivity and a detection limit of 0.4U/L owing to the low background. The detection of ALP in human serum was conducted with satisfactory results, demonstrating its potential applications in clinical diagnosis.
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