期刊
TALANTA
卷 175, 期 -, 页码 189-193出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.07.041
关键词
Circulating tumor cells; Aptamer; Proteome; Open tubular column
资金
- National Key Research and Development Program [2016YFA0501401]
- National Natural Science Foundation [21575143, 21375128, 21235005]
Circulating tumor cells hold the key to predicting the prognosis and discovering the therapeutic targets. Herein, we proposed a strategy to develop an aptamer-immobilized open tubular capillary column by which SMMC-7721 human hepatoma cells (SMMC-7721 cells) could be captured with an over 70% of capture efficiency and a 3.0 +/- 0.2 of enrichment factor. Owing to the compatibility of the column, the captured cells by the column could be analyzed by LC-MS from protein level and 5 unique proteins of SMMC-7721 cells were identified which could be used as markers to identify SMMC-7721 cells when Jurkat T-leukemia cells (Jurkat cells) were employed as interfering cells. As the key component, the aptamer-immobilized column had the potential to be integrated into the platform for separating, enriching and characterizing rare cells simultaneously.
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