期刊
TALANTA
卷 168, 期 -, 页码 279-285出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.03.014
关键词
Biosensor; Aptamer; Gold nanoparticles; Colorimetric detection
资金
- Ministry of Science and Technology of China [2012ZX09506001-010]
- National Natural Science Foundation of China [21302108]
A simple and sensitive colorimetric aptasensor for rapid and facile detection of adenosine triphosphate (ATP) has been demonstrated here based on aptamer-based hairpin probes and unmodified gold nanoparticles (AuNPs). The hairpin probe is constructed by adding another five nucleotides to the 5'-end of an anti-ATP aptamer which can hybridize to nucleotides at the 3'-end of the aptamer, forming a hairpin-shaped structure. In the absence of ATP, the hairpin probes are rigid, and the AuNPs are susceptible to salt-induced aggregation. Conversely, upon binding with target ATP, the hairpin probes undergo conformational changes, forming aptamer-ATP complexes and exposing flexible ends which coat the surface of AuNPs to inhibit their aggregation in the high salt solution. Subsequently, a blue-to-red color change can be recognized by the naked eye. The aptasensor achieved selective responses toward ATP with a detection limit of 0.1 mu M, and exhibited high-quality detection performance in biological samples. In addition, this detection method is simple, rapid and cost-effective, holding great potential for further applications in point-of-care research.
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