期刊
TALANTA
卷 170, 期 -, 页码 502-508出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.04.054
关键词
Progesterone; Biotinylated antibody; Immunosensor; Thionine-graphene oxide composites; Milk samples
资金
- National Natural Science Foundation of Guangdong Province [2015030313793, 2014A030306026]
- Science and Technology Foundation of Guangdong Province [2016A020210102, 2014TQ01N109]
- Excellent Young Teachers Program on Higher Education of Guangdong Province [2014145, Y920/4026]
Progesterone (P4) is a kind of hormone that can cause neuropathic disturbances in humans when the concentration overpasses a certain degree. In this work, an electrochemical immunosensor capable of detecting P4 sensitively and selectively was developed. Thionine-graphene oxide (Thi-GO) composites with excellent biocompatibility were synthesized and coated to a clear glassy carbon electrode. P4 coating antigen (P4-OVA) was immobilized to the electrode, then sample as well as biotinylated antibody (biotin-P4 Ab) were added. The free P4 can compete with P4-OVA for binding to biotin-P4 Ab. After the further addition of streptavidin-HRP, H2O2 was introduced to develop electrical signal for quantitative determination of P4. After careful optimization of assay conditions, the proposed immunosensor showed a linear range from 0.02 to 20ngmL(-1) for P4 in milk samples. The averaged recoveries from spiked samples ranged from 84.0% to 102.0%, which correlated well with standard HPLC-MS/MS. The biosensor also showed good specificity, reproducibility and stability, indicating its potential application in monitoring of P4 in a simple and low cost manner.
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