Ga(III) complex with morin for kidney cancer cell labelling

The formation of a complex between Ga(III) and morin (3,5,7,2', 4'-pentahydroxyflavone) was studied. UV-visible, infrared and mass spectroscopies were used to characterize the complex. The stoichiometric ratio for the reaction between metal ion and flavonoid was determined using the methods of Yoe-Jones and Job, which confirmed that a 1: 1 Ga-morin complex was formed (estimated binding constant = 2.31 x 10(4) l mol(-1)). It was found that the coordination to Ga(III) occurs through the carbonyl oxygen atom and the 3-OH group of the morin molecule. According to developed conditions, complexation reaction with Ga-68 was performed and the complex was used to label kidney cancer cells (CAKI-1, CAKI-2, ACHN and 786-O). The knowledge gained from this study should be useful for the development of new radiopharmaceuticals for diagnostic purposes containing Ga-68.