Development of a real-time PCR method for quantification of Prevotella histicola from the gut

We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 10(3) colony-forming units (CFU) in the gut. (C) 2017 Elsevier Ltd. All rights reserved.