期刊
ACTA VIROLOGICA
卷 61, 期 4, 页码 483-486出版社
AEPRESS SRO
DOI: 10.4149/av_2017_411
关键词
BoHV-1; virus isolation; VP16; CPE
类别
资金
- Chinese National Science Foundation Grant [31472172]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Priority Academic Program Development of Jiangsu Higher Education Institutions (TAPP)
- Jiangsu province [BE2014358, 14KJA230001]
- Agricultural Science and Technology Innovation Program [ASTIP-IAS-11]
Bovine herpesvirus 1 (BoHV-1) infection causes substantial economic losses to the cattle industry worldwide. So far, the isolation of BoHV-1 field virus has not been reported in China. Here, for the first time we report that two isolates of BoHV-1 designated as NJ16-1 and NJ16-2 were obtained from semen samples from breeding bulls in China. Typical cytopathic effect in MDBK cells, detection of viral protein VP16 in western blot analysis, PCR detection of BoHV-1 gB gene proved BoHV-1 infection and subsequent nucleotide sequence analysis showed a 99% identity with BoHV-1 Cooper strain. These results suggest that these isolated viruses are BoHV-1.
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