期刊
STEM CELLS AND DEVELOPMENT
卷 26, 期 16, 页码 1141-1161出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2017.0055
关键词
naive human pluripotency; inner cell mass; human embryonic stem cell; hESC; blastocyst; epiblast
资金
- NIH/NHLBI [U01HL099775]
- NIH/NEI [R01EY023962]
- NIH/NICHD [R01HD082098]
- RPB Stein Innovation Award
- Maryland Stem Cell Research Fund [2013-MSCRF-III-114936, 2013-MSCRFII-0032-00, 2014-MSCRFE-118153]
- Novo Nordisk Science Forum Award
Although human embryonic stem cells (hESCs) were first derived almost 20 years ago, it was only recently acknowledged that they share closer molecular and functional identity to postimplantation lineage-primed murine epiblast stem cells than to naive preimplantation inner cell mass-derived mouse ESCs (mESCs). A myriad of transcriptional, epigenetic, biochemical, and metabolic attributes have now been described that distinguish naive and primed pluripotent states in both rodents and humans. Conventional hESCs and human induced pluripotent stem cells (hiPSCs) appear to lack many of the defining hallmarks of naive mESCs. These include important features of the naive ground state murine epiblast, such as an open epigenetic architecture, reduced lineage-primed gene expression, and chimera and germline competence following injection into a recipient blastocyst-stage embryo. Several transgenic and chemical methods were recently reported that appear to revert conventional human PSCs to mESC-like ground states. However, it remains unclear if subtle deviations in global transcription, cell signaling dependencies, and extent of epigenetic/metabolic shifts in these various human naive-reverted pluripotent states represent true functional differences or alternatively the existence of distinct human pluripotent states along a spectrum. In this study, we review the current understanding and developmental features of various human pluripotency-associated phenotypes and discuss potential biological mechanisms that may support stable maintenance of an authentic epiblast-like ground state of human pluripotency.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据