Generation of induced pluripotent stem cells from peripheral blood CD34+hematopoietic progenitors of a 31 year old healthy woman

The MUi019- A human induced pluripotent stem cell line was generated from peripheral blood CD34+ hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019- A to model disease while the cell type affected by the disease is inaccessible. (C) 2017 The Authors. Published by Elsevier B.V.