期刊
STEM CELL RESEARCH
卷 19, 期 -, 页码 118-127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scr.2017.01.005
关键词
HLA-G; hESC Promoter methylation; 3 ' UTR; MicroRNA
资金
- Wetenschappelijk Fonds Willy Gepts of the UZ Brussel [WFWG08-10, WFWG13-20]
- research committee of the Vrije Universiteit Brussel [OZR1447, 1447 BOF]
- Department of Hematology, Universitair Ziekenhuis Brussel (UZ Brussel)
The human leukocyte antigen (HLA)-G gene seems to play a pivotal role in maternal tolerance to the fetus. Little is known about HLA-G expression and its molecular control during in vivo human embryogenesis. Human embryonic stem cells (hESC) provide an interesting in vitro model to study early human development. Different studies reported discrepant findings on whether HLA-G mRNA and protein are present or absent in hESC. Several lines of evidence indicate that promoter CpG methylation and 3' untranslated region (3'UTR) polymorphisms may influence HLA-G expression. We investigated how HLA-G expression is linked to the patterns of promoter methylation and explored the role of the 3'UTR polymorphic sites and their binding microRNAs on the post-transcriptional regulation of HLA-G in eight hESC lines. We showed that, while the gross expression levels of HLA-G are controlled by promoter methylation, the genetic constitution of the HLA-G 3'UTR, more specifically the 14bp insertion in combination with the + 3187A/A and + 3142G/G SNP, plays a major role in HLA-G mRNA regulation in hESC. Our findings provide a solid first step towards future work using hESC as tools for the study of early human developmental processes in normal and pregnancy-related disorders such as preeclampsia. (C) 2017 The Authors. Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据