期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 170, 期 -, 页码 104-110出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2016.07.018
关键词
Umbelliferone; Scopoletin; Excitation-emission matrix fluorescence; Second-order calibration; Alternating trilinear decomposition
类别
资金
- National Natural Science Foundation of China [21575039]
- National Basic Research Program of China [2012CB910602]
- Foundation for Innovative Research Groups of NSFC [21521063]
A chemometrics-assisted excitation-emission matrix (EEM) fluorescence method is presented for simultaneous determination of umbelliferone and scopoletin in Tibetan medicine Saussurea laniceps (SL) and traditional Chinese medicine Radix angelicae pubescentis (RAP). Using the strategy of combining EEM fluorescence data with second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm, the simultaneous quantification of umbelliferone and scopoletin in the two different complex systems was achieved successfully, even in the presence of potential interferents. The pretreatment is simple due to the second-order advantage and the use of mathematical separation instead of awkward physical or chemical separation. Satisfactory results have been achieved with the limits of detection (LODs) of umbelliferone and scopoletin being 0.06 ng mL(-1) and 0.16 ng mL(-1) respectively. The average spike recoveries of umbelliferone and scopoletin are 98.8 +/- 4.3% and 102.5 +/- 33%, respectively. Besides, HPLC-DAD method was used to further validate the presented strategy, and t-test indicates that prediction results of the two methods have no significant differences. Satisfactory experimental results imply that our method is fast, low-cost and sensitive when compared with HPLC-DAD method. (C) 2016 Elsevier B.V. All rights reserved.
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