期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 175, 期 -, 页码 164-167出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2016.12.033
关键词
ATP; Label-free; Aptasensor; Thioflavin T; G-quadruplex formation
类别
资金
- National Natural Science Foundation of China [21205142, 31370104]
- Research Innovation Program for Graduates of Central South University [2016zzts580]
The present work demonstrates a simple, rapid and label-free ATP detection method using a fluorescent aptasensor that is based on G-quadruplex formation. In the absence of ATP, the Thioflavin T (ThT) dye binds to the G-rich ATP aptamer and forms an ATP aptamer/ThT G-quadruplex complex, which results in high fluorescence intensity. Upon addition of ATP, the ATP aptamer/rhT complex will be replaced by the formation of an ATP aptamer/ATP complex. During this process, separation of the ThT dye from the ATP aptamer/ThT complex decreases the fluorescence intensity of the reaction mixture dramatically. This fluorescence aptasensor is highly sensitive and rapid, with a detection limit of 18 nM and a total reaction time of only 10 min. Furthermore, this method is cost-effective and simple, removing the requirement for labeling the detection reagents with a fluorophore-quencher pair. (C) 2016 Elsevier B.V. All rights reserved.
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