期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 77, 期 -, 页码 38-45出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2015.05.006
关键词
GH61; AA9; Lytic polysaccharide monooxygenase; Lignocellulose bioconversion; Bioethanol
资金
- National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2010-0020141]
- Forest Science & Technology Projects, Forest Service, Republic of Korea [S211314L010120]
- Korea Forest Service [S211314L010120] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Lignocellulose is a renewable resource that is extremely abundant, and the complete enzymatic hydrolysis of lignocellulose requires a cocktail containing a variety of enzyme groups that act synergistically. The hydrolysis efficiency can be improved by introducing glycoside hydrolase 61 (GH61), a new enzyme that belongs to the auxiliary activity family 9 (AA9). GH61 was isolated from Gloeophyllum trabeum and cleaves the glycosidic bonds on the cellulose surface via oxidation of various carbons. In this study, we investigated the properties of GH61. GtGH61 alone did not exhibit any notable activity, but the synergistic activity of GtGH61 with xylanase (GtXyl10G) or cellulase (GtCel5B) showed efficient bioconversion rates of 56 and 174% in pretreated kenaf (Hibiscus cannabinus L.) and oak (Quercus spp.), respectively. Furthermore, the GtGH61 activity was strongly accelerated in the presence of cobalt Co2+. Enzyme cocktails (GtXyl10G, GtCel5B, and GtGH61) increased the amount of sugar released by 7 and 6% for pretreated oak and kenaf, respectively, and the addition of Co2+ stimulated bioconversion by 12 and 11% in pretreated oak and kenaf, respectively. (C) 2015 Elsevier Inc. All rights reserved.
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