期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 73-74, 期 -, 页码 9-19出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2015.03.001
关键词
Fusarium commune; Peptide pattern recognition; Transcriptome; Glycoside hydrolase; Xylanase; Pichia pastoris
资金
- Sino-Danish Center (SDC)
- Danish Strategic Research Council [2101-08-0041, 09-065251]
- Novozymes A/S
Specific enzymes from plant-pathogenic microbes demonstrate high effectiveness for natural lignocellulosic biomass degradation and utilization. The secreted lignocellulolytic enzymes of Fusarium species have not been investigated comprehensively, however. In this study we compared cellulose and hemicellulose-degrading enzymes of classical fungal enzyme producers with those of Fusarium species. The results indicated that Fusarium species are robust cellulose and hemicellulose degraders. Wheat bran, carboxymethylcellulose and xylan-based growth media induced a broad spectrum of lignocellulolytic enzymes in Fusarium commune. Prediction of the cellulose and hemicellulose-degrading enzymes in the F. commune transcriptome using peptide pattern recognition revealed 147 genes encoding glycoside hydrolases and six genes encoding lytic polysaccharide monooxygenases (AA9 and AM 1), including all relevant cellulose decomposing enzymes (GH3, GH5, GH6, GH7, GH9, GH45 and M9), and abundant hemicellulases. We further applied peptide pattern recognition to reveal nine and seven subfamilies of GH10 and GH11 family enzymes, respectively. The uncharacterized XYL10A, XYL1OB and XYL11 enzymes of F. commune were classified, respectively, into GH10 subfamily 1, subfamily 3 and GH11 subfamily 1. These xylanases were successfully expressed in the PichiaPink (TM) system with the following properties: the purified recombinant XYL10A had interesting high specific activity; XYL1OB was active at alkaline conditions with both endo-1,4-beta-D-xylanase and beta-xylosidase activities; and XYL11 was a true xylanase characterized by high substrate specificity. These results indicate that F. commune with genetic modification is a promising source of enzymes for the decomposition of lignocellulosic biomass. (c) 2015 Elsevier Inc. All rights reserved.
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