Molecular characterization and expression analysis of CSαβ defensin genes from the scorpion Mesobuthus martensii

Defensins are important components of innate host defence system against bacteria, fungi, parasites and viruses. Here, we predicted six potential defensin genes from the genome of the scorpion Mesobuthus martensii and then validated four genes from them via the combination of PCR and genomic sequence analysis. These four scorpion defensin genes share the same gene organization and structure of two exons and one phase-I intron with the GT-AG rule. Conserved motif and phylogenetic analysis showed that they belonged to the members of the invertebrate cysteine-stabilized alpha-helix/beta-sheet motif defensin (CS alpha beta) defensin family. All these four CS alpha beta defensin genes have the expression feature of constitutive transcription (CON) by the whole scorpion infection model, promoter sequence analysis and dual luciferase assays. Further evolution and comparison analysis found that the invertebrate CS alpha beta defensin genes from most of arachnids and mollusks appear to share the expression pattern of CON, but those from insects and lower invertebrates (nematodes, annelids, cnidarians and sponges) seem to have identical inducible transcription (IND) after being challenged by microorganisms. Together, we identified four scorpion CS alpha beta defensin genes with the expression feature of CON, and characterized the diversified expression patterns of the invertebrate CS alpha beta defensin genes, which will shed insights into the evolution of the invertebrate CS alpha beta defensin genes and their expression patterns.