期刊
ADVANCED FUNCTIONAL MATERIALS
卷 27, 期 45, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201702748
关键词
base-excision repair; bioimaging; DNA circuit; MnO2 nanosheet; nanoassembly
类别
资金
- National Science Foundation of China [21475102, 31671013]
- Fundamental Research Funds for the Central Universities [xjj2017039]
- Young Talent Support Plan of Xi'an Jiaotong University
Nanomaterial/DNA integrated systems have become an emerging tool for intracellular imaging. However, intracellular catalytic DNA circuit is rarely explored. Commonly used nanosystems neglect intracellular DNA assembly, conformation folding and catalytic efficiency, all demanding appropriate metal ion conditions. Herein, MnO2 nanosheet/DNAzyme (nanozyme) is fabricated as intracellular catalytic DNA circuit generator for high signal amplification, and its operation is reported for monitoring DNA base-excision repair (BER) in living cells with improved performance. MnO2 nanosheet works as not only DNA nanocarrier but also as DNAzyme cofactor supplier. The nanozyme is constructed by adsorbing DNA probes on MnO2 nanosheets, facilitating cellular uptake of DNA. They are rapidly released in cellular environments by reducing MnO2 nanosheets to Mn2+ as DNAzyme cofactor. After repair enzyme activation, nanozymes are properly assembled with active folded conformation and hold sustained catalytic efficiency over many cycles. It offers at least 40-fold amplified signals for the monitoring of apurinic/apyrimidinic endonuclease-initiated and DNA glycosylase-initiated BER pathways. Multiplex imaging can be allowed by integrating several sets of probes with per MnO2 nanosheet. The MnO2 nanozyme opens up exciting opportunities for imaging low-abundance biomarkers and relevant biological pathways in living cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据