期刊
SMALL
卷 14, 期 9, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201703152
关键词
delivery; HCC; nanoassemblies; RNA; supramolecular
类别
资金
- National Key Research and Development Program of China [2016YFC1100404, 2016YFA0201501, 2015AA020950]
- National Natural Science Foundation of China [51325304, 51473014, 51521062, 31671300]
- Taishan Scholars Program of Shandong Province [tsqn20161060]
The delivery of tumor-suppressive noncoding RNAs (ncRNAs) including short ncRNAs (i.e., miRNAs) and long ncRNAs (lncRNAs) is put forward to treat tumors. In this work, novel rodlike supramolecular nanoassemblies (CNC@CB[8]@PGEA) of degradable poly(aspartic acid) (PAsp) derivatives-grafted cellulose nanocrystals (CNCs) and hydroxyl-rich polycations (ethanolamine-functionalized poly(glycidyl methacrylate), PGEA) are proposed via typical cucurbit[8]uril (CB[8])-based host-guest interactions for delivery of different ncRNAs to treat hepatocellular carcinoma (HCC). Spindly CNCs, one kind of natural polysaccharide nanoparticles, possess good biocompatibility and unique physico-chemical properties. PGEA with abundant hydroxyl groups is one promising gene carrier with low cytotoxicity. PAsp can benefit the disassembly and degradability of nanoassemblies within cells. CNC@CB[8]@PGEA combines the different unique properties of CNC, PGEA, and PAsp. CNC@CB[8]@PGEA effectively complexes the expression constructs of miR-101 (plasmid pc3.0-miR-101) and lncRNA MEG3 (plasmid pc3.0-MEG3). CNC@CB[8]@PGEA produces much better transfection performances than PGEA-containing assembly units. In addition, the codelivery system of CNC@CB[8]@PGEA/(pc3.0-MEG3+pc3.0-miR-101) nanocomplexes demonstrates better efficacy in suppressing HCC than CNC@CB[8]@PGEA/pc3.0-MEG3 or CNC@CB[8]@PGEA/pc3.0-miR-101 nanocomplexes alone. Such rodlike supramolecular nanoassemblies will provide a promising means to produce efficient delivery vectors of versatile tumor-suppressive nucleic acids.
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