期刊
HELIYON
卷 3, 期 7, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2017.e00348
关键词
Microbiology; Food science
资金
- Alberta Livestock Meat Agency (ALMA)
- Alberta Innovates - Bio Solutions (Al Bio)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were determined. The minimum inhibitory concentration of glycated hydrolysates decreased by 1.2 times compared to the native hydrolysate, with no differences between (+) or (-) TGase treatments. No difference was observed in the dicarbonyl compound concentration between the two glycation methods except that 3-deoxyglucosone was greater in the TGase-mediated reaction. Concanavalin A-retentate, but not the flow-through fractions, significantly improved the antimicrobial activity, however there was no difference between +TGase and -TGase glycated treatments. Purification of the retentate fraction from fluorescent compounds did not improve its antimicrobial activity.
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