Fluorescence completely separated ratiometric probe for HClO in lysosomes

Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), was reported to be involved in a number of human diseases and many efforts have therefore been made to probe its biological roles at molecular level. In this work, a ratiometric probe (BFCIO) with completely separated fluorescent signals consisting of a boron-dipyrromethene (BODIPY) dye conjugated with Fisher aldehyde.was designed and synthesized for the selective and sensitive detection of HOCI via oxidative cleavage of the alkene linker. The probe evinced more than 300-fold enhancement in fluorescence ratio(I-511nm/I-713nm) in the presence of HClO over other ROS with fast response time (within 10 s) and low detection limit (10.6 nM). Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane and visualize exogenous and endogenous HOCI in lysosomes of living cells. (C) 2017 Elsevier B.V. All rights reserved.