期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 238, 期 -, 页码 249-256出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.07.073
关键词
Biosensor; Porous Co3O4 hexagonal nanosheets; Direct electron transfer; Nitrite
资金
- National Science Foundation of China [51272147]
- Natural Science Foundation of Shaanxi Province [2015JM5208]
- Graduate Innovation Found of Shaanxi University of Science and Technology
Porous Co3O4 hexagonal nanosheets (PCHNSs) with a well-defined morphology have been synthesized successfully via a hydrothermal process and followed by calcinations. Co3O4 nanosheets immobilizing horseradish peroxidase (HRP), together with reduced graphene oxide, have been used to modify glassy carbon electrode to fabricate a mediator-free biosensor. The morphology and structure of PCHNSs are studied by XRD, FESEM, and HRTEM techniques. Spectroscopic and electrochemical results reveal that Co3O4/rGO composite film is an excellent immobilization matrix with biocompatibility for enzymes, affording good enzymes' bioactivity and stability. Meanwhile, due to the unique structure of the modified electrode, the direct electron transfer of HRP is facilitated and the prepared nitrite biosensors display good performance a wide linear range of 1-5400 mu M, an extremely low limit of detection (LOD) as 0.21 mu M and a limit of quantification as (LOQ) as 0.58 mu M. This work suggests that Co3O4/rGO composite film is a potential matrix for enzymes' immobilization and biosensors' preparation. (C) 2016 Elsevier B.V. All rights reserved.
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