期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 252, 期 -, 页码 209-214出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.009
关键词
Aptamer; Fluorescence; Cu NCs; PKA activity
资金
- National Natural Science Foundation of China [21275063, 21575048]
- science and technology development project of Jilin province, China [20150204010GX]
Herein, we firstly used double-strand-templated DNA Cu nanoclusters (Cu NCs) as a simple, lable-free and sensitive fluorescence (FL) probe to detect protein kinase (PKA) activity. This method was based on the strong interaction between adenosine triphosphate (ATP) aptamer and ATP. When ATP was added, fluorescent Cu NCs could not be formed due to the lack of effective substrate and the fluorescence of Cu NCs decreased. However, when PKA was added, the fluorescence of Cu NCs recovered because that ATP had been translated into ADP by PKA and ADP can not combine with ATP aptamer. We can effective monitored the activity of protein kinase according to the variation of fluorescence signal in the range of 0.1-1000 mU/mu L. The detection limit (LOD) is 0.041 mU/mu L. We also used this method to detect protein kinase inhibitor H-89. In addition, this method was also used to explore the activity of drug-induced PKA in Hela cells, which makes the sensor had a great application value in biochemistry and targeted kinase drug discovery research. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据