期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 253, 期 -, 页码 115-123出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.108
关键词
Antibody-carbon nanotube bio-conjugate; Staphylococcus aureus; Paper substrate; Electrochemical immunosensor; Label-free detection
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [2015R1A2A2A01006446]
- National Research Foundation of Korea [2015R1A2A2A01006446] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The need for low-cost, sensitive, and reliable sensors for the detection of whole bacterial cells in food samples without pre-treatment has been increasing. Outbreaks of foodborne diseases can be severe, especially in developing countries; however, most bio-detection tools are unaffordable. Here, we have developed a rapid and low-cost paper-based electrochemical immunosensor for label-free detection of Staphylococcus aureus, using antibody (Ab)-single walled carbon nanotube (SWCNT) bio-conjugates. Anti-S. aureus antibodies were covalently attached onto the SWCNTs, using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide coupling reagent. These Ab-SWCNT bio-conjugates were then immobilized on the working electrode, and the presence of S. aureus was detected by analyzing the change in peak current following antigen-antibody complex formation. Differential pulse voltammetry was performed with a bacterial concentration ranging from 10 to 10(7) colony forming units (CFU) mL(-1). A selectivity assay using Escherichia coli B, Bacillus subtilis, and S. epidermidis (to examine cross-reactivity) showed that the sensor was specific to S. aureus. Moreover, this immunosensor showed a good linear relationship (R-2 = 0.976) between the increase in peak current and logarithmic S. aureus concentration, with a rapid detection time (30 min) and a limit of detection of 13 CFU mL(-1) in spiked milk samples. This low-cost immunosensor can be used for rapid detection of pathogens in actual food samples with high sensitivity and specificity. (C) 2017 Elsevier B.V. All rights reserved.
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