期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 247, 期 -, 页码 546-555出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.03.079
关键词
Listeria monocytogenes; Vancomycin; Magnetic nanoparticles; PCR; Detection
资金
- Research Foundation for Young Scientists of State Key Laboratory of Food Science and Technology, Nanchang University, China [SKLF-QN-201504]
- National Natural Science Foundation of China [31271863]
In this study, vancomycin (Van) functionalized PEGylated-magnetic nanoparticles (Van-PMs) combined with polymerase chain reaction (PCR) showed great potential for highly efficient detection of L. monocytogenes at low infectious dose. Herein, Van-PMs played as universal molecular probes for targeting bacterial cells, while PEG was used to help Van close to D-alanyl-D-alanine (D-Ala-D-Ala) moieties on surface of L. monocytogenes. Under optimum conditions, the developed Van-PMs complexes exhibited extremely high capture efficiency (CE) in a 10 mL bacterium suspension with the value was higher than 90% and 83% in the phosphate-buffered saline (PBS) solution and lettuce samples, respectively. Meanwhile, Gram-negative bacteria slightly influenced CE even under concentrations as high as 10(6) CFU/mL. The assay of Van-PMs-PCR was applied to detect L. monocytogenes with detection limits of 30 CFU/mL and 30 CFU/g in PBS solution and lettuce samples, respectively. The whole experiment was completed within 4 h. This assay demonstrated the potential of this modified method in practical application involving the detection of L. monocytogenes or other Gram-positive pathogenic bacteria. (C) 2017 Elsevier B.V. All rights reserved.
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