期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 252, 期 -, 页码 44-54出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.05.148
关键词
Paper-based microfluidics; Electrochemical immunosensor; Reduced graphene oxide-tetraethylene pentamine; Screen-printed electrode; Point-of-care testing
资金
- National Key Technologies R & D Program of China [2013BAI03B01]
- National Natural Science Foundation of China [61627807, 61271119]
- Scientific and Technical Development Project from the Education Department of Guangxi [2013YB090]
- Hunan Provincial Innovation Fund for Postgraduate [CX2014B043]
- Guangxi Natural Science Foundation [2015GXNSFAA139032]
A novel, disposable and sensitive microfluidic paper-based electrochemical immunosensor (mu-PEI) was developed by using gold nanoparticles (Au NPs) decorated reduced graphene oxide-tetraethylene pentamine (rGO-TEPA/Au nanocomposite) as electrode materials. The rGO-TEPA greatly amplified the current response due to its excellent conductivity and large surface area. Au NPs was decorated on the surface of rGO-TEPA to increase the biocompatibility and retained good stability for rGO-TEPA/Au nanocomposite, which was used as an effective sensor platform for anchoring the capturing antibodies (Ab]) and accelerating the electron transfer to the screen-printed electrodes (SPEs). The simple and disposable paper-based microfluidic channel patterned on the rGO-TEPA/Au nanocomposite modified SPEs (SPEs/rGO-TEPA/Au) was designed for combination of immunochromatography and immunofiltration simultaneously. With the combination of portable SPEs/rGO-TEPA/Au and simple paper-based microfluidic devices, horseradish peroxidase (HRP) and labeled signal antibodies (Ab(2)) co-immobilized gold nanorods (HRP-GNRs-Ab(2)) was explored as the tracers for square wave voltammetry (SWV) detection. Using Alpha-Fetoprotein (AFP) as a model analyte, the proposed mu-PEI exhibited a satisfactory performance like simple fabrication, high stability, selectivity, wide linear range (0.01 ng mL(-1) -100.0 ng mL(-1)) with a low detection limit (0.005 ng mL(-1)). Furthermore, human serum were applied to the obtained mu-PEI and determined with satisfactory results. (C) 2017 Published by Elsevier B.V.
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