期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 243, 期 -, 页码 745-752出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.12.031
关键词
Naloxone; Electrochemical sensor; Molecularly imprinted polymer; Multi-walled carbon nanotubes; Screen printed electrode
资金
- FCT-Fundacao para a Ciencia e a Tecnologia for a Pos-Doc [SFRH/BPD/101419/2014]
- POPH-QREN-Tipologia 4.1-Formacao Avancada
- Fundo Social Europeu and Ministerio da Ciencia, Tecnologia e Ensino Superior
- European Union (FEDER funds through COMPETE)
- National Funds (FCT) [UID/QUI/50006/2013]
Naloxone (NLX) is a pharmaceutical used as opioid antagonist. A molecular imprinted polymer electrochemical sensor for simple and rapid detection of NIX was prepared through the modification of commercial available screen printed carbon electrode (SPCE). The SPCE was modified with multi-walled carbon nanotubes (MWCNT) by drop coating to increase the signal response and improve the sensitivity. The MIP preparation was carried out via in situ electropolymerization using 4-aminobenzoic acid (4-ABA) as functional monomer. The morphology of the obtained sensor was characterized by scanning electron microscopy (SEM). Several parameters controlling the preparation and performance of the MIP sensor were studied and optimized. The electrochemical behavior of NIX at MIP and control non imprinted (NIP) sensor was evaluated by differential pulse voltammetry (DPV), demonstrating a better MIP response and the success of the imprinting. The proposed MIP/MWCNT/SPCE sensor showed a linear relationship between peak current intensity and NIX concentration in the range between 0.25 and 10.0 mu M, with limits of detection (LOD) and quantification (LOQ) of 0.20 mu M and 0.67 mu M respectively. The repeatability and reproducibility were also tested with relative standard deviations (RSD) of 4.6 and 9.6% respectively. Moreover, the applicability of the method was successfully confirmed with detection of NLX in biological samples (urine and human serum). The sensor is promising to be used for screening NIX in point-of-care people with opioid overdose. (C) 2016 Elsevier B.V. All rights reserved.
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