期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 251, 期 -, 页码 804-812出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.05.122
关键词
Reduced graphene oxide; Biosensor; Bisphenol A; Horseradish peroxidase; Gold nanoparticles
资金
- Basic Science Research Program [2016R1A2B4013374]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning (MSIP), Korea [2015M2A2A6A02045262(3), 2017M2A2A6A01020938]
- Technology Innovation Program - Ministry of Trade, Industry&Energy (MI, Korea) [10049587]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10049587] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2015M2A2A6A02045262, 2016R1A2B4013374] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In the present study, we employ a straightforward, benign strategy to prepare thiol-functionalized reduced graphene oxide (S-RGO) using methionine as the sulphur source and reducing agent. The immobilization of horseradish peroxidase (HRP) over the AuNPs/S-RGO was developed by incorporating AuNPs on the S-RGO surface. The fabricated HRP/AuNPs/S-RGO electrode exhibits a remarkable decrease in the overpotential and a significantly increased oxidation peak current of bisphenol A (BPA) compared with the bare glassy carbon electrode (GCE) and AuNPs/S-RGO electrode. The biosensor shows an excellent amperometric analytical performance with a low detection limit of 2.6 x 10(-12) M and a linear range from 2.0 x 10(-11) to 1.18 x 10(-9) M, with the response time <2 s for BPA. From the results, the apparent Michaelis-Menten constant was calculated as 8.14 nM. The HRP/AuNPs/S-RGO biosensor exhibited faster response, adequate storage stability, inexpensive, simple fabrication with disposability, satisfactory reproducibility and repeatability, and outstanding selectivity. Finally, the constructed biosensor was utilized successfully for detecting BPA in tomato juice and milk samples with acceptable results. (C) 2017 Elsevier B.V. All rights reserved.
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