Ultrasensitive QDs based electrochemiluminescent immunosensor for detecting ractopamine using AuNPs and Au nanoparticles@PDDA-graphene as amplifier

In this work, gold nanoparticals (AuNPs) and CdSe QDs decorated poly (diallyldimethylamrnonium chloride)-graphene (CdSe QDs/PDDA-GN/AuNPs) were prepared and applied as probe to assemble an electrochemiluminescence (ECL) immunosensor for detecting ractopamine (RAC) for the first time. The CdSe QDs/PDDA-GN/AuNPs composite showed good electronic conductivity, fast response and satisfactory stability which employed for fabricating ECL biosensors with high sensitivity. AuNPs and PDDA-GN were creatively applied in this sensor not only accelerate electron conduction to achieve dual signal amplification, but also provide large surface for coating antigen and antibody loading. A competitive immunoassay strategy was chosen for determining RAC, where RAC in the sample would compete with the coating antigen for the limited biomarkers. The well-prepared ECL immunosensor satisfied in samples detection of RAC with a wider linear range from 0.01 to 1000 ngmL(-1) and a lower detection limit of 2.6 pg mL(-1) (S/N =3). The proposed ECL immunosensor displayed high sensitivity and stability, and provided an approach for determining other important biomarkers. (C) 2016 Elsevier B.V. All rights reserved.