期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 242, 期 -, 页码 710-717出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.11.063
关键词
Centrifugal microfluidics; Conditional siphon priming; Ammonium analysis
资金
- National Natural Science Foundation of China [31500691, 81471749, 81327005]
- Beijing Municipal Science & Technology Commission [Z161100000116031]
- Beijing Lab Foundation
Centrifugal microfluidics has proven to be successful in biomedical diagnostics, biological analysis and environmental monitoring. However, the automation of multi-step sample processing, reaction and detection remains a great challenge. In this study, a conditional siphon priming technique is introduced for multi-step liquid addition or selective routing. Since the siphon channel is locally modified or venting is blocked by liquid in another chamber, siphon priming can be triggered by liquid addition or venting at low frequency. Using this technique, sequential release,of liquids and selective routing in multiple manners were successfully achieved. As a proof of concept, a centrifugal microfluidic platform was designed for on-site ammonium analysis in water samples. The linear range of ammonium concentrations is extended by integration of a dilution process. This novel valving technique provides new solutions for integration of complex liquid handling processes on centrifugal platforms. (C) 2016 Elsevier B.V. All rights reserved.
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