期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 246, 期 -, 页码 943-951出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.02.151
关键词
Gold nanocluster; 3,4-Dihydroxy-L-phenyl alanine (L-DOPA); Fluorescent sensor; Antioxidant; Neurotransmitter; Ascorbic acid
资金
- Department of Science and Technology, Government of India [IF 150393]
- Department of Chemistry, University of Kerala, Thiruvananthapuram, India
The design of biocompatible fluorescent probes for targeting vital molecules in brain is highly desirable in recent years since various statistical studies conducted by World Health Organization point out the upcoming risk of neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. In this circumstance, we synthesized water soluble fluorescent 3, 4-dihydroxy-L-phenyl alanine (L-DOPA) protected gold nanocluster (L-DOPA-AuNC) as L-DOPA can be effectively transmitted through blood -brain barrier. The as prepared L-DOPA-AuNCs exhibited fluorescence emission at 545 nm. Interestingly, no obvious change in fluorescence emission of L-DOPA-AuNC was observed over a broad pH range (3 to 10), in various buffer solutions (10 mM phosphate buffer), or in solutions with high ionic strength(2 M KCl). However, the unique co-ordination of Fe(III) ions with catechol group on the surface of L-DOPAAuNCs induce fluorescence quenching. Moreover, the fluorescence of L-DOPA-AuNCs can be regained completely by antioxidants (biological reducing agents) like Ascorbic Acid (AA) with high selectivity over other antioxidants, amino acids and neurotransmitters. The Limit of Detection (LOD) for Fe (III) ion and AA were found to be 25.5 zM and 18.4 pM respectively. Furthermore, human serum spiked with Fe (III) ions and AA is analyzed using this sensor and show good recovery percentage. (C) 2017 Elsevier B.V. All rights reserved.
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