期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 245, 期 -, 页码 1032-1038出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.01.185
关键词
Cholesterol sulfate; Sulfated lipids; Steroids; Chemical sensors; Methinium salts; Troger's bases
资金
- Ministry of Education, Youth and Sports of the Czech Republic within the National Sustainability Program II (Project BIOCEV-FAR) [LQ1604]
- projects BIOCEV [CZ.1.05/1.1.00/02.0109, LH 14008]
- Charles University in Prague [UNCE 204011, Progres Q26/LF1, Progres Q27/LF1]
- GACR [14-36804G]
- MZCR [RVO-VFN64165/2012]
Methinium colorimetric sensors 1 and 2 were studied for the determination of cholesterol sulfate by UV-Vis spectroscopy in 0.001 M NaCl (H2O:MeOH; 7:3), pH = 7.34. Both of the tested sensors displayed high affinity, excellent selectivity and sensitivity for cholesterol sulfate against other steroids (estrone sulfate, pregnenolone sulfate, cholesterol, cholic acid and taurocholic acid). The determined values of conditional binding constants, linear ranges and detection limits demonstrated the high usability of these sensors for the determination of cholesterol sulfate. For example, the detection limits of tested sensors I and 2 for the detection of cholesterol sulfate were 0.16 mu M (0.07 mg/L; R-2 = 0.9966) and 1.3 mu M (0.59 mg/L; R-2 = 0.9872), respectively. Our detection limit strongly indicates the potential usability of these sensors for the determination of biologically important levels of cholesterol sulfate. (C) 2017 Elsevier B.V. All rights reserved.
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