期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 244, 期 -, 页码 142-150出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.12.132
关键词
Heavy metal ion detection; Phytochelatins; Porous silicon; QCM; Corrosion
资金
- Italian National Operative Programs [PON01_01525, PON 04a2_F]
Phytochelatins (PCs), oligomers of glutathione, naturally chelate heavy metals (HMs) in aqueous solution; small peptides such as these cannot be used as covalently bound bioprobes on transducer surfaces due to their propensity to induce corrosion of standard sensor supports such as gold and porous silicon (PSi). In this work, we chemically modify a commercial PC oligopeptide with a six poly-lysine (Lys) chain, thereby changing its isoelectric point from 4.2 to 6.9. PC-Lys bioprobes were successfully immobilized on both PSi multilayers and flat gold surfaces. The interaction of PC-Lys and HM ions, namely Lead (II), Cadmium (II) and Arsenic (III) in aqueous solution was quantified by optical spectroscopic reflectometry and quartz crystal microgravimetry. As a result, it was proven that the biomolecular interactions are reversible and the affinities between PC-Lys complex and HM ions are in the range of 10(-12) M. (C) 2016 Elsevier B.V. All rights reserved.
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