期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 253, 期 -, 页码 470-477出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.153
关键词
ECL; Immunosensor; MoS2-PEI-Au nanocomposites; Au@BSA core/shell nanoparticles; AFP
资金
- National Natural Science Foundation of China [21373138]
- Shanghai Education Committee [15ZZ070]
- International Joint Laboratory on Resource Chemistry (IJLRC)
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_16R49]
- Science and Technology Innovation Foundation for College Students from Shanghai Normal University
In this work, a novel and universal sandwich-type electrochemiluminescence (ECL) immunosensor was constructed for the sensitive and specific detection of alpha fetal protein (AFP), in which the MoS2 nanosheets surface was modified using polyethylenimine (PEI) polymer with plentiful amino-groups (MoS2-PEI) for warranting their positively charged interface and thereby a large amount of negatively charged gold nanoparticles (AuNPs) were electrostatically adsorbed to form MoS2-PEI-Au nanocomposites. Meanwhile, the synthesized Au@BSA nanoparticles were utilized to immobilize molecule recognition probe secondary antibodies (Ab(2)) and signal probe numerous luminol molecules, respectively. Upon the optimization of assay conditions, the proposed immunosensor displayed wide linear range from 0.0001 ng/mL to 200.0 ng/mL with limit of detection of 1.0 x 10(-5) ng/mL. Moreover, this immunosensor was employed to analyze AFP in real human serum samples with favorable results. The presented immunosensing strategy may provide great potential in the assay of other clinically biomarkers. (C) 2017 Elsevier B.V. All rights reserved.
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