期刊
SENSORS
卷 17, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/s17040856
关键词
localized surface plasmon resonance (LSPR); optical interferometry; anodic aluminum oxide (AAO); pore-size; pore-area; serum amyloid A1 (SAA1); optical-biosensor; C-reactive protein (CRP)
资金
- National Research Foundation of Korea (NRF) - Korea Government (MSIP) [NRF 2014R1A2A1A11050377]
- BK21 Plus project - Ministry of Education, Korea [21A20131600011]
In this study, we developed a pore size/pore area-controlled optical biosensor-based anodic aluminum oxide (AAO) nanostructure. As the pore size of AAO increases, the unit cell of AAO increases, which also increases the non-pore area to which the antibody binds. The increase in the number of antibodies immobilized on the surface of the AAO enables effective detection of trace amounts of antigen, because increased antigen-antibody bonding results in a larger surface refractive index change. High sensitivity was thus achieved through amplification of the interference wave of two vertically-incident reflected waves through the localized surface plasmon resonance phenomenon. The sensitivity of the fabricated sensor was evaluated by measuring the change in wavelength with the change in the refractive index of the device surface, and sensitivity was increased with increasing pore-size and non-pore area. The sensitivity of the fabricated sensor was improved and up to 11.8 ag/mL serum amyloid A1 antigen was detected. In addition, the selectivity of the fabricated sensor was confirmed through a reaction with a heterogeneous substance, C-reactive protein antigen. By using hard anodization during fabrication of the AAO, the fabrication time of the device was reduced and the AAO chip was fabricated quickly and easily.
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