期刊
SENSORS
卷 17, 期 9, 页码 -出版社
MDPI AG
DOI: 10.3390/s17092128
关键词
gold nanoparticles; interdigitated electrodes; electrochemical-enzymatic redox cycling; covalent immobilization; cholesterol sensor
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2016R1A2B4010031]
- UNIST [1.170013.01]
- Ministry of Science & ICT (MSIT), Republic of Korea [2017미래선도형] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2016R1A2B4010031, 22A20130000116] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs. Gold nanoparticle characteristics such as size and morphology were reproducibly controlled by modulating the step-potential and time period in the electrodeposition processes. To detect cholesterol selectively using AuNP/carbon IDEs, cholesterol oxidase (ChOx) was selectively immobilized via the electrochemical reduction of the diazonium cation. The sensitivity of the AuNP/carbon IDE-based biosensor was ensured by efficient amplification of the redox mediators, ferricyanide and ferrocyanide, between selectively immobilized enzyme sites and both of the combs of AuNP/carbon IDEs. The presented AuNP/carbon IDE-based cholesterol biosensor exhibited a wide sensing range (0.005-10 mM) and high sensitivity (similar to 993.91 mu A mM(-1) cm(-2); limit of detection (LOD) similar to 1.28 mu M). In addition, the proposed cholesterol biosensor was found to be highly selective for the cholesterol detection.
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