期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 149, 期 -, 页码 174-183出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.10.022
关键词
Giant vesicles; Erythrocyte; Lipid membrane; Cytoskeleton reconstitution; AFM; PeakForce tapping quantitative nanomechanical mapping
资金
- ERC [ERC-StG-2013-883188]
- Programa Ramon y Cajal from Spanish Ministry of Economy MINECO [RYC-2013-12609]
- ERC NANOFORCELLS [ERC-StG-2011-278860]
- FORCE-for-FUTURE [CSD2010-00024]
- MINECO [FIS2012-35723]
Erythrocyte membranes have been particularly useful as a model for studies of membrane structure and mechanics. Native erythroid membranes can be electroformed as giant unilamellar vesicles (eGUVs). In the presence of ATP, the erythroid membrane proteins of eGUVs rearrange into protein networks at the microscale. Here, we present a detailed nanomechanical study of individual protein microfilaments forming the protein networks of eGUVs when spread on supporting surfaces. Using Peak Force tapping Atomic Force Microscopy (PF-AFM) in liquid environment we have obtained the mechanical maps of the composite lipid-protein networks supported on solid surface. In the absence of ATP, the protein pool was characterized by a Young's Modulus E-pool approximate to 5-15 MPa whereas the complex filaments were found softer after protein supramolecular rearrangement; E-fil approximate to 0.4 MPa. The observed protein softening and reassembling could be relevant for understanding the mechanisms of cytoskeleton reorganization found in pathological erythrocytes or erythrocytes that are affected by biological agents. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licensesiby-nc-nd/4.0/).
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