Improving Bread Quality with the Application of a Newly Purified Thermostable -Amylase from Rhizopus oryzae FSIS4

A new thermostable -amylase from Rhizopus oryzae FSIS4 was purified for first time and recovered in a single step using a three-phase partitioning (TPP) system. The fungal -amylase, at a concentration of 1.936 U per kg of flour, was used in bread-making and compared to the commercial enzyme. The results showed a significant effect of the recovered -amylase in the prepared bread and allowed us to improve the quality of the bread. The study indicated clearly that the recovered -amylase is a potential candidate for future applications in the bread-making industry and in other food biotechnology applications.