期刊
BIORESOURCE TECHNOLOGY
卷 223, 期 -, 页码 105-114出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.10.024
关键词
Piscirickettsia salmonis; Genome-scale metabolic model; Metabolic reconstruction; Nutritional requirement; Defined medium
资金
- FONDEF project [D10I1185]
- PhD scholarship CONICYT of Ministry of Education, Chile Government
Piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. This pathology is partially controlled by vaccines. The lack of knowledge has hindered its culture on laboratory and industrial scale. The study describes the metabolic phenotype of P. salmonis in culture. This study presents the first genome-scale model (iPF215) of the LF-89 strain of P. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mechanisms. The model was adjusted with experiment data, allowing the identification of the capacities that were not predicted by the automatic annotation of the genome sequences. The iPF215 model is comprised of 417 metabolites, 445 reactions and 215 genes, was used to reproduce the growth of P. salmonis (mu(max) 0.052 +/- 0.005 h(-1)). The metabolic reconstruction of the P. salmonis LF-89 strain obtained in this research provides a baseline that describes the metabolic capacities of the bacterium and is the basis for developing improvements to its cultivation for vaccine formulation. (C) 2016 Elsevier Ltd. All rights reserved.
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