期刊
SCIENTIA HORTICULTURAE
卷 219, 期 -, 页码 118-124出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2017.02.050
关键词
Postharvest banana fruits; HPLC; Energy level; Adenylate concentrations; Senescence; Chilling injury
类别
资金
- National Natural Science Foundation of China [31160407, 31560467, 31660589, 31560006]
- Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China [Ren She Ting Han [2015]192]
- Guangxi Natural Science Foundation [2014GXNSFDA118013, 2015GXNSFBA139102]
- Guangxi Agricultural Key Science and Technology Program [201527]
- Guangxi Scientific Research and Technological Development Projects [Gui Ke AD16380015]
- Foundation of Fundamental Research Project from Guangxi Academy of Agricultural Sciences [2015YT86, 2015JM14, 2015JZ75, 2014YQ05, 2016JZ11]
- Special Fund for Bagui Scholars [[2016]21]
Energy level is closely related to postharvest banana fruit senescence. In this study, high performance liquid chromatography (HPLC) method was developed to determine concentrations of adenylate, i.e. adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP), in postharvest banana fruits. Boiling water extracting adenylate from banana fruits was suitable because of its high extraction rate and stability. HPLC method exhibited good repeatability (variation coefficients of 1.51%-3.42%) and recovery rate (93.9%-97.8%). The correlation coefficients of ATP, ADP and AMP with peak areas in a range of 1-120 mg/L were 0.999965, 0.999995 and 0.999996, respectively. Through analyzing adenylate concentrations and membrane hydrolysis-related enzyme activities (phospholipase D and lipoxygenase, ab. PLD and LOX, two key enzymes catalyzing membrane lipid degradation) in banana fruits stored at different temperatures (7 degrees C, 14 degrees C and 25 degrees C), it could be found that appropriate low temperature (14 degrees C) delayed fruit senescence by maintaining high energy level. Chilly stress (7 degrees C) accelerated fruit senescence by declining energy supply level, accompanying by activation of membrane hydrolysis related enzymes such as PLD and LOX. These results were helpful for elucidating relationship between energy metabolism and senescence regulation during chilling injury in postharvest banana fruits. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据