期刊
SCIENTIA HORTICULTURAE
卷 214, 期 -, 页码 174-179出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2016.11.033
关键词
Agrobacterium-mediated transformation; Citrus maxima; Dark incubation; in planta transformation
类别
资金
- International Science & Technology Cooperation Program of China [2012DFA30610]
- Guangdong Provincial Science and Technology Programs [2016B020201006, 2014B020202009, 2014B070706018]
A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integration confirmation and transgenic plant propagation. The highest transformation efficiency (20.41%) was obtained when using hygromycin as selection marker, followed by Basta (1937%) and Kanamycin (3.21%). Trans genic plants obtained using this method could be graft-propagated in 3 months post transformation, which is shorter than the tissue-culture-dependent transformation. The transformation protocol presented here has great potential to be used for citrus genetic breeding and improvement. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据