期刊
SCIENCE TRANSLATIONAL MEDICINE
卷 9, 期 392, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scitranslmed.aaf6295
关键词
-
资金
- Fidelity Biosciences Research Initiative (F-PRIME)
- NIH/National Institute on Aging [R21 AG044713]
- BrightFocus Foundation Fellowship
- Edward R. and Anne G. Lefler Fellowship
The complement cascade not only is an innate immune response that enables removal of pathogens but also plays an important role in microglia-mediated synaptic refinement during brain development. Complement C3 is elevated in Alzheimer's disease (AD), colocalizing with neuritic plaques, and appears to contribute to clearance of Ab by microglia in the brain. Previously, we reported that C3-deficient C57BL/6 mice were protected against age-related and region-specific loss of hippocampal synapses and cognitive decline during normal aging. Furthermore, blocking complement and downstream iC3b/CR3 signaling rescued synapses from Ab-induced loss in young AD mice before amyloid plaques had accumulated. We assessed the effects of C3 deficiency in aged, plaque-rich APPswe/PS1dE9 transgenic mice (APP/PS1; C3 KO). We examined the effects of C3 deficiency on cognition, Ab plaque deposition, and plaque-related neuropathology at later AD stages in these mice. We found that 16-month-old APP/PS1; C3 KO mice performed better on a learning and memory task than did APP/PS1 mice, despite having more cerebral Ab plaques. Aged APP/PS1; C3 KO mice also had fewer microglia and astrocytes localized within the center of hippocampal Ab plaques compared to APP/PS1 mice. Several proinflammatory cytokines in the brain were reduced in APP/PS1; C3 KO mice, consistent with an altered microglial phenotype. C3 deficiency also protected APP/PS1 mice against agedependent loss of synapses and neurons. Our study suggests that complement C3 or downstream complement activation fragments may play an important role in Ab plaque pathology, glial responses to plaques, and neuronal dysfunction in the brains of APP/PS1 mice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据