期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 48, 期 1, 页码 71-78出版社
SOC BRASILEIRA MICROBIOLOGIA
DOI: 10.1016/j.bjm.2016.09.007
关键词
Heavy metal contamination; PCR-DGGE fingerprinting; Multivariate analysis; Bacterial community structure
类别
资金
- program for National Natural Science Foundation of China [31471812, 31171809]
- Fundamental Research Funds forhangsu Academy of Agricultural Sciences [ZX(15)4007]
In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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