期刊
BIOMATERIALS
卷 115, 期 -, 页码 115-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.11.018
关键词
3D electrospun nanofibrous scaffold; Polycaprolactone; Polylactic acid; Osteogenic differentiation; Bone regeneration
资金
- EPSCoR program of National Science Foundation [IIA-1335423]
- Competitive Research Grant program of South Dakota Board of Regents [UP1500172]
- COBRE grants of National Institutes of Health [P20 GM103620, P20 GM103548]
- CAPES Foundation of the Ministry of Education, Brazil [99999.010045/2014-07]
- Office of Integrative Activities
- Office Of The Director [1355423] Funding Source: National Science Foundation
Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of similar to 95.8% as well as interconnected and hierarchically structured pores with sizes from sub-micrometers to similar to 300 mu m for bone tissue engineering. The hypothesis was that the incorporation of PLA (with higher mechanical stiffness/modulus and bioactivity) into PCL nanofibers would significantly improve human mesenchymal stem cells (hMSCs) osteogenic differentiation in vitro and bone formation in vivo. Compared to neat PCL-3D scaffolds, PCL/PLA-3D blend scaffolds had higher mechanical properties and in vitro bioactivity; as a result, they not only enhanced the cell viability of hMSCs but also promoted the osteogenic differentiation. Furthermore, our in vivo studies revealed that PCL/PLA-3D scaffolds considerably facilitated new bone formation in a critical-sized cranial bone defect mouse model. In summary, both in vitro and in vivo results indicated that novel 3D electrospun PCL/PLA blend nanofibrous scaffolds would be strongly favorable/desired for hMSCs osteogenic differentiation and cranial bone formation. (C) 2016 Elsevier Ltd. All rights reserved.
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