期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 135, 期 -, 页码 40-47出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2016.09.011
关键词
Herbicides; HA; Zebrafish; Tissues; CYP 1A; EROD
资金
- Major Science and Technology Program for Water Pollution Control and Treatment [2012ZX07501-003]
Humic acid (HA) is a major component of dissolved organic matter, is ubiquitous in the aquatic environment and influences the biological toxicity of organic pollutants. In this study, we investigated the cytochrome P450 IA (CYP 1A) mRNA expression and ethoxyresorufin-O-deethylase (EROD) activity in the gills and liver of zebrafish following exposure to the s-triazine herbicide prometryn with or without HA present. Prometryn induced both CYP 1A mRNA expression and EROD activity. The CYP lA mRNA expression of zebrafish that were exposed to a combination of prometryn and HA was increased compared to those exposed to prometryn alone. A likely cause for CYP 1A induction is the impact of special components of HA, functioning as aryl hydrocarbon receptor (AHR) agonists. In combination with HA, these increase prometryn levels in tissues. Similar results for EROD activity were evident. In our time course study, CYP lA mRNA expression reached maximum values during 24 h. This revealed CYP lA mRNA transcription as a comparatively sensitive toxicity index. In a recovery experiment, we found a faster decrease of CYP lA mRNA expression to control levels (CK) in gills compared to liver tissue. Following exposure to HA, CYP 1A mRNA expression in liver tissue displayed a faster decrease to CK levels. HA induced enhanced metabolic rates for prometryn. In contrast, recovery regularity of CYP 1A expression in gills was independent of the presence of HA. This result indicates different detoxification mechanisms for HA in liver and gills. (C) 2016 Elsevier Inc. All rights reserved.
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