期刊
PHOTOMEDICINE AND LASER SURGERY
卷 36, 期 2, 页码 83-91出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/pho.2017.4344
关键词
photobiomodulation; high-intensity laser therapy; Nd:YAG; equine mesenchymal stem cells; IL-10; VEGF
类别
Objective: This study aimed to examine the influence of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on equine bone marrow-derived mesenchymal stem cell (MSC) viability, proliferation, and cytokine expression in vitro. Background: Photobiomodulation of cells using monochromatic light is a technique designed to influence cellular processes. Previous studies have shown dose-dependent effects of low-level laser irradiation on cell proliferation and cytokine expression in a range of cell types and species. Evidence for the influence of 1064nm wavelength near-infrared irradiation on MSCs is sparse, and high-energy doses have shown inhibitory effects. Methods: MSC cultures from six horses were exposed to 1064nm irradiation with an energy density of 9.77J/cm(2) and a mean output power of 13.0W for 10sec. MSC viability and proliferation were evaluated through flow cytometry and real-time live cell analysis. Gene expression and cytokine production in the first 24h after irradiation were analyzed through polymerase chain reaction (PCR), multiplex assay, and enzyme-linked immunosorbent assay. Results: No difference in viability was detected between irradiated and control MSCs. Irradiated cells demonstrated slightly lower proliferation rates, but remained within 3.5% confluence of control cells. Twenty-four hours after irradiation, irradiated MSCs demonstrated a significant increase in expression of interleukin (IL)-10 and vascular endothelial growth factor (VEGF) compared with control MSCs. Conclusions: Under these irradiation parameters, equine MSCs remained viable and expressed increased concentrations of IL-10 and VEGF. IL-10 has an anti-inflammatory action by inhibiting the synthesis of proinflammatory cytokines at the transcriptional level. This response to 1064nm irradiation shows promise in the photobiomodulation of MSCs to enhance their therapeutic properties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据