期刊
BIOLOGICAL CHEMISTRY
卷 399, 期 1, 页码 47-54出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2017-0187
关键词
degradomics; parallel reaction monitoring; protease substrates; selected reaction monitoring; targeted proteomics
资金
- Swiss National Science Foundation [31003A_163216]
- Swiss National Science Foundation (SNF) [31003A_163216] Funding Source: Swiss National Science Foundation (SNF)
Targeted degradomics integrates positional information into mass spectrometry (MS)-based targeted proteomics workflows and thereby enables analysis of proteolytic cleavage events with unprecedented specificity and sensitivity. Rapid progress in the establishment of protease-substrate relations provides extensive degradomics target lists that now can be tested with help of selected and parallel reaction monitoring (S/PRM) in complex biological systems, where proteases act in physiological environments. In this minireview, we describe the general principles of targeted degradomics, outline the generic experimental workflow of the methodology and highlight recent and future applications in protease research.
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