期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 22, 期 17, 页码 13049-13057出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-015-4545-0
关键词
Biodegradation; Catabolites; beta-Cypermethrin; Extracellular enzyme; Pseudomonas aeruginosa GF31
资金
- National Natural Science Foundation of China [51108098]
- Graduate Student Innovation Projects of Guangxi [GXU11T31090]
- Scientific Research Foundation of Guangxi University [XJZ120278]
Pseudomonas aeruginosa strain GF31, isolated from a contaminated soil, can effectively degrade beta-cypermethrin (beta-CP), as well as fenpropathrin, fenvalerate, and cyhalothrin. The highest level of degradation (81.2 %) was achieved with the addition of peptone. Surprisingly, the enzyme responsible for degradation was mainly localized to the extracellular areas of the bacteria, in contrast to the other known pyrethroid-degrading enzymes, which are intracellular. Although intact bacterial cells function at about 30 A degrees C for biodegradation, similar to other degrading strains, the crude extracellular extract of strain GF31 remained biologically active at 60 A degrees C. Moreover, the extract fraction showed good storage stability, maintaining > 50 % of its initial activity following storage at 25 A degrees C for at least 20 days. Significant differences in the characteristics of the crude GF31 extracellular extract compared with the known pyrethroid-degrading enzymes indicate the presence of a novel pyrethroid-degrading enzyme. Furthermore, the identification of 3-phenoxybenzoic acid and 2,2-dimethylcyclopropanecarboxylate from the degradation products suggests the possibility that beta-CP degradation by both the strain and the crude extracellular fraction is achieved through a hydrolysis pathway. Further degradation of these two metabolites may lead to the development of an efficient method for the mineralization of these types of pollutants.
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