期刊
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 79, 期 1, 页码 -出版社
WILEY
DOI: 10.1111/aji.12783
关键词
Ccl25; Ccr9; chemokine; ectoplacental cone; endometrium; fetal-maternal interface
资金
- FAPESP
- CAPES
- CNPq
ProblemWe hypothesized that trophoblast expression of Ccl25 attracts a specific leukocyte cell population to the implantation site for local regulation. Method of studyMice blastocysts, ectoplacental cones, and decidua at gestational days 3.5-7.5 were evaluated for Ccl25 and Ccr9 expressions. Peripheral availability and characterization of Ccr9+ leukocytes were determined by flow cytometry. Leukocyte chemotaxis was assessed in the presence of Ccl25 recombinant protein and embryos using antisense oligomers (ODNs) to Ccl25 and Ccr9 neutralizing antibody. ResultsCcl25 was expressed by embryonic cells, whereas Ccr9 expression was strong at the maternal compartment and in PBMC. Immunolocalization confirmed this expression. In vitro, chemotaxis assays showed that the embryonic Ccl25 signals to Ccr9+ PBMCs. Maternal Ccr9+47+ monocytes switch from an anti-inflammatory phenotype (F4/80+11b+Ly6C-TGF-+ cells, pre-implantation) to an inflammatory profile (F4/80+11b+Ly6C+TNF-+ cells, post-implantation). ConclusionOur data support the establishment of a CCL25/CCR9-axis at the maternal-fetal interface in mice, which may be involved in immune regulatory mechanisms during embryo implantation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据